Improvement of PolyCYPs® Microbial Cytochrome P450 Cell Factory for Efficient Whole-Cell Biotransformation
Presented at: Synthetic Biology 2019, Warwick UK, joint with scientists at John Ward‘s group at University College London.
PolyCYP6, a functional bacterial Class I cytochrome P450, was successfully mined from one of Hypha’s actinomycete strains and converts the non-steroidal anti-inflammatory drug diclofenac into its hydroxylated human metabolites.
Improvement of the PolyCYP cytochrome P450 cell factory for efficient whole-cell biotransformation was achieved through co-expression with specific redox partners and changing components of the T7 expression plasmid. The highest yielding recombinant strain expressed PolyCYP6 with the P. putida reductase system. An ampicillin resistant marker & high copy vector also further improved yields of hydroxylated products.