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Improvement of PolyCYPs® Microbial Cytochrome P450 Cell Factory for Efficient Whole-Cell Biotransformation

Improvement of PolyCYPs® Microbial Cytochrome P450 Cell Factory for Efficient Whole-Cell Biotransformation

Presented at: Synthetic Biology 2019, Warwick UK, joint with scientists at John Ward‘s group at University College London.

Scientist doing an experimentPolyCYP6, a functional bacterial Class I cytochrome P450, was successfully mined from one of Hypha’s actinomycete strains and converts the non-steroidal anti-inflammatory drug diclofenac into its hydroxylated human metabolites.

Improvement of the PolyCYP cytochrome P450 cell factory for efficient whole-cell biotransformation was achieved through co-expression with specific redox partners and changing components of the T7 expression plasmid. The highest yielding recombinant strain expressed PolyCYP6 with the P. putida reductase system. An ampicillin resistant marker & high copy vector also further improved yields of hydroxylated products.

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