Launch of PolyUGTs Kits
Our PolyUGTs screening and scale-up kits are now available! They’re a super easy way to make glucuronides for MetID and testing in your own labs.
Hypha’s PolyUGT enzymes are mined from selected biotransforming strains in Hypha’s microbial collection, expressed in E.coli, and subsequently purified. The enzymes provided in the screening kit have been tested for biocatalytic activity against a number of substrates known to be subject to glucuronidation. This includes glucuronidation of alkyl and phenolic hydroxyls for O-glucuronides, carboxylic acids for acyl-glucuronides, as well as aromatic nitrogen-containing systems for N-glucuronides. Glucuronidation of some non-aromatic alkyl N-moieties is also observed.
Currently 11 PolyUGT isoforms are available in a screening kit and scale-up kit format for clients to use in their own labs. Hit reactions can be scaled up to obtain material for structure elucidation by NMR to pinpoint the position of attachment, biological activity assessment, validation of sample handling processes for bioanalysis, and for in vitro drug-drug interaction testing.
Read more about the kits.
